WebThe circular dichroic spectra of α-globulin fromSesamum indicum L. was recorded in the presence of cetyltrimethyl ammonium bromide, Triton X-100 and Brij-36T. The protein in … Webtion was obtained with Brij 36T, Brij 56, and Brij 96 at a detergent:protein weight ratio of 4:l and with octaethylene glycol dodecyl ether at a ratio of 2:l. Similar Ca2+-induced crystalline arrays were obtained with the purified or delipidated Ca2+-ATPase of sar- coplasmic reticulum but at lower detergent:protein ra- tios.
Solubilization of a stereospecific opiate-macromolecular complex …
WebMay 15, 1987 · Microcrystalline arrays of Ca2+-transporting ATPase (EC 3.6.1.38) develop in detergent-solubilized sarcoplasmic reticulum upon exposure to 10-20 mM CaCl2 at pH 6.0 for several weeks at 2 degrees C, in a crystallization medium that preserves the ATPase activity for several months. WebJun 1, 1981 · After sensitization with IgG, the integral membrane proteins were solubilized in Brij 36T nonionic detergent and chromatographed by gel filtration. There was a distinct resolution of IgG into... monarch butterfly optimization
Solubilization of a stereospecific opiate-macromolecular complex …
WebTreatment of crude gonococcal cell envelopes with a solution of 2 M KCl + 1% Brij 36T resulted in the solubilization of a portion of the D-alanine carboxypeptidase activity of Neisseria ... WebOf 48 detergents tested, optimal crystallization was obtained with Brij 36T, Brij 56, and Brij 96 at a detergent:protein weight ratio of 4:1 and with octaethylene glycol dodecyl ether at a ratio of 2:1. Similar Ca2+-induced crystalline arrays were obtained with the purified or delipidated Ca2+-ATPase of sarcoplasmic reticulum but at lower ... WebDec 1, 1980 · The supernatant was removed with a pasteur pipet and centrifuged once more at 100,000 X g for 15 min. This supernatant was dialyzed against 50 volumes of Tris-Brij 36T buffer (50 mm TrisHC1 buffer containing 1% Brij 36T and 0.04% sodium azide) for 5 hr with buffer changes every hour. iasip season 6