Webcollection into the pLX304-Blast-V5 vector to create the CCSB-Broad Lentiviral Expression Library (Fig. 2a). We conducted a pilot experiment on 509 ORF clones to assess: (i) protocols to transfer the WebThe CCSB–Broad Lentiviral Expression Library is a genome-scale expression collection developed by researchers at Dana-Farber Cancer Institute and The Broad Institute …

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WebA public genome-scale lentiviral expression library of human ORFs Authors: Xiaoping Yang 2 , Jesse S Boehm 8 , Xinping Yang 1 , 3 , 7 , Kourosh Salehi-Ashtiani 1 , 3 , 4 ... WebDo the CCSB-Broad Lentiviral Expression clones have a native stop codon? No, all of the clones in the CCSB-Broad Lentiviral Expression Library contain a C-terminal V5 epitope tag fused to the ORF of interest. There is a stop codon located at the 3' end of the V5 epitope tag (Figure 3). How do I package the CCSB-Broad Lentiviral Expression clones? esszencia szinonima

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WebThe CCSB-Broad Lentiviral Expression Library for human open reading frames. Choose from over 15,00 sequence-confirmed CMV-driven human ORFs with a C-terminal V5 tag … WebJun 26, 2011 · We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector … WebJun 26, 2011 · Performance of the CCSB-Broad lentiviral expression library. (a) Micrographs show A549 lung cancer cell lines stained with an antibody to the V5 epitope after lentiviral infection and 3 d of ... esszénusok