2024 Chondrocyte affinity peptide

Chondrocyte affinity peptide

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August undefined, 2024

WebSep 1, 2024 · Through plasmids transfection, liang et al. (Liang et al., 2024) fused chondrocyte-affinity peptide (CAP) with EV surface protein, and improved the chondrocyte target ability of CAP-EVs. Apart from protein fusion, regulating EV surface charge potential can promote EV penetration since the block influence of negative … WebOct 1, 2024 · Chondrocyte affinity peptide (CAP) with sequence DWRVIIPPRPSA identified using phage display effectively delivered Hif-2α siRNA to chondrocytes when …

Chondrocyte affinity peptide modified PAMAM conjugate as a ... - PubMed

WebNov 8, 2024 · Pi et al. identified a short CHP sequence with a high binding affinity toward chondrocytes. They introduced a non-viral vector in which fluorescently labeled chondrocyte-affinity peptide (CAP) was covalently bound to polyethyleneimine (PEI) and injected into rabbit knees to target hyaline cartilage. WebKeywords: extracellular vesicle, exosome, chondrocyte ... Exosome retention in the articular cavity was improved by fusing a chondrocyte-affinity peptide on the exosome surface. The peptide confined the exosomes to the articular cavity and facilitated exosome penetration into the middle zone of the cartilage tissue to achieve targeted ... crit nasus build

Modification of mesenchymal stem cells for cartilage-targeted …

WebMar 12, 2024 · Materials & methods: Chondrocyte affinity peptide modified PEGylated polyamidoamine conjugates (CAP-PEG-PAMAM) were prepared and rhodamine B … WebChondrocyte-targeting hybrid exosomes We first developed chondrocyte-targeting exosomes, which contain a surface-displayed chondrocyte-affinity peptide (CAP) through genetic engineering of the exosomal membrane protein, lysosomal-associated membrane protein 2b (Lamp2b) in dendritic cells (DC). WebNov 23, 2024 · The chondrocyte-affinity peptide (CAP) was identified, and it was suggested that this ligand could specifically bind to chondrocytes [ 8 ]. Although its binding receptor has not yet been elucidated, the peptide is considered as an excellent candidate for chondrocyte targeting. crit museum parker az

National Center for Biotechnology Information

WebMay 1, 2024 · Controlling chondrocyte senescence. New research published in Science Translational Medicine implicates microRNA-204 in the mechanism by which oxidative stress results in altered chondrocyte ... WebApr 21, 2024 · The patient’s MRI procedure first confirms a surgery-worth defect. A chondrocyte sample is taken via an arthroscopic procedure. New chondrocytes are … buffalo nas ls210d 初期化WebBy fusing a chondrocyte-affinity peptide (CAP) with the lysosome-associated membrane glycoprotein 2b protein on the surface of exosomes, we acquire CAP-exosomes that can efficiently encapsulate miR-140, specifically enter, and deliver the cargo into chondrocytes in vitro. CAP-exosomes, in contrast to nontagged exosome vesicles, are retained in ... crit nation parker az

"WebIn our study, we identified a chondrocyte-affinity peptide (CAP, DWRVIIPPRPSA) by phage display technology. Subsequent analysis suggests that the peptide can efficiently … " - Chondrocyte affinity peptide

Chondrocyte affinity peptide

National Center for Biotechnology Information

WebDec 3, 2015 · Cell-adhesive ligands or affinity peptides provide a more effective method for cell recruitment of biomaterial scaffolds 19,20. Arginine-glycine-aspartic acid is a well … WebWe displayed a chondrocyte-affinity peptide (CAP) on the pIII minor coat protein of the M13 filamentous bacteriophage (phage)-based particle carrying a mammalian transgene cassette under cytomegalovirus CMV promoter and inverted terminal repeats (ITRs) cis elements of adeno-associated virus serotype 2 (AAV-2). Primary human articular ...

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WebNov 13, 2024 · The affinity of DPI peptides towards BMSCs was analyzed quantitatively by flow cytometry following incubation with FITC-labeled LSP, DPI or RGD. The average … WebAlthough still in its infancy, exosome-mediated drug delivery boasts low toxicity, low immunogenicity, and high engineerability, and holds promise for cell-free therapies for a …

WebApr 29, 2024 · Recently, a senolytic peptide, fork head box O transcription factor 4-D-Retro-Inverso (FOXO4-DRI), was reported to selectively kill the senescent fibroblasts. In this study, we hypothesized that FOXO4-DRI treatment could remove the senescent cells in the expanded chondrocytes, thus enhancing their potential in generating high-quality cartilage. WebJun 21, 2024 · Methods: Chondrocyte-targeting exosomes (CAP-Exo) were constructed by genetically fusing a chondrocyte affinity peptide (CAP) at the N-terminus of the exosomal surface protein Lamp2b.

WebMay 21, 2024 · Synovial fluid derived MSCs transplantation is an effective method to treat OA cartilage degeneration. kartogenin (KGN) is a small molecule that can induce MSCs to differentiate into chondrocytes in vitro and in vivo. It controls the chondrogenic differentiation of transplanted MSCs. WebFeb 15, 2024 · In addition to chondrocyte affinity peptides, MSC affinity peptides can be used to construct cell-free scaffolds used to repair cartilage defects, where they recruit autologous MSCs. Ao et al. developed several MSC affinity peptides and used them to repair cartilage defects [36-39]. E7 is a peptide screened by phage display with a high …

WebFeb 15, 2024 · The use of peptides in cartilage tissue engineering, which utilizes a combination of growth factors, cells, and scaffolds. In terms of growth factors, TGF-β …

WebApr 1, 2024 · Download Citation On Apr 1, 2024, Xinning Yu and others published Cartilage-targeting mRNA-lipid nanoparticles rescue perifocal apoptotic chondrocytes for integrative cartilage repair Find ... crit number plateWebSaid integrin a9β1 ligand has a higher binding affinity with integrin a9β1 than tenascin-C and osteopontin , ... comprising an antibody against osteopontin or fragment peptide portion thereof as an active ingredient. ... promoting chondrocyte differentiation, inhibiting cartilage calcification and inhibiting cartilage degradation, ... buffalo nas ls210do301 remote cloud webaccessWebBinding assays substantiated that the affinity K d values of four designed cyclic peptides (, , and ) were improved to 23, 67, 42 and 18 μM, respectively, from the 96 μM of linear eILP peptide. Keywords: Chondrocyte senescence; Conformational flexibility; Cyclization; Matrix metallopeptidase 13; Osteoarthritis; Ration peptide design; Self ... buffalo nas ls220de firmwareWebMar 12, 2024 · Materials & methods: Chondrocyte affinity peptide modified PEGylated polyamidoamine conjugates (CAP-PEG-PAMAM) were prepared and rhodamine B … buffalo nas ls210 passwordWebNov 8, 2024 · Pi et al. identified a short CHP sequence with a high binding affinity toward chondrocytes. They introduced a non-viral vector in which fluorescently labeled … buffalo nas ls220d0802gWebApr 16, 2015 · A phage display technique was used to identify a chondrocyte-affinity peptide (CAP; DWRVIIPPRPSAC), which is a potential therapeutic agent with better transfection efficiency and … buffalo nas ls220d firmwareWebMay 1, 2024 · Controlling chondrocyte senescence. New research published in Science Translational Medicine implicates microRNA-204 in the mechanism by which oxidative stress results in altered chondrocyte ... buffalo nas ls420d0802 download