Extended protein occupancy domains
WebJun 25, 2024 · The waa locus forms an extended protein occupancy domain (EPOD), a long region of bacterial chromosome characterized by unusually dense levels of DNA … WebDec 28, 2024 · E. coli extended protein occupancy domains (EPODs) are densely occupied and transcriptionally silent genomic regions resembling eukaryotic …
Extended protein occupancy domains
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WebThere are several different types of protein domains. Head Domain: The Head domain consists of the start codon followed immediately by zero or more triplets specifiying an N … WebJan 30, 2024 · To address this challenge, we have developed high-resolution in vivo protein occupancy display (IPOD-HR), a technology that enables rapid, quantitative, and comprehensive monitoring of DNA-protein interactions across a bacterial chromosome.
WebDiscovery of Extended Protein Occupancy Domains Intriguingly, examination of the entire genome-wide occupancy profile revealed contiguous regions of protein binding, many of which extend beyond a kilobase in length (Figures 3A–3D and S5). We performed a systematic search for these extended protein occupancy domains (EPODs) under … WebI show that these regions, termed extended protein occupancy domains (EPODs), have functional roles in bacteria that enable them to use new carbon sources for energy and provide an immune defense against viruses in Escherichia coli (E. coli). I show that EPODs are occupied by nucleoid associated proteins (NAPs).
WebJun 25, 2024 · A 5-kb rolling median is plotted. ChIP, chromatin immunoprecipitation; EPOD, extended protein occupancy domain; IPOD-HR, in vivo protein occupancy … WebApr 11, 2024 · A novel procedure for the application of atom probe tomography (APT) to the structural analysis of biological systems, has been recently proposed, whereby the specimen is embedded by a silica matrix and ablated by a pulsed laser source. Such a technique, requires that the silica primer be properly inert and bio-compatible, keeping the native …
WebJan 29, 2024 · We observe that the E. coliprotein-DNA interactome organizes into two distinct prototypic features: (1) highly dynamic condition- dependent transcription factor occupancy, and (2) robust kilobase scale occupancy by nucleoid 30 factors, forming silencing domains analogous to eukaryotic heterochromatin.
WebI show that these regions, termed extended protein occupancy domains (EPODs), have functional roles in bacteria that enable them to use new carbon sources for energy and … pin board wall coveringWebJul 1, 2009 · Protein occupancy profiling experiments have demonstrated the presence of heterochromatin-like domains termed extended protein occupancy domains (EPODs) across many regions of the E. coli ... to prepare ethyne and examine its propertiesWebAt a larger scale, the authors also found contiguous regions of protein binding that extended to over 1,000 bp in length, which they termed extended protein occupancy … pin board warehouseWebIn this report, we focused our study on the role of HU protein, a nucleoid-associated protein referred to as a histone-like protein, in the nucleoid compaction of D. radiodurans. We demonstrate, using a new system allowing conditional gene expression, that HU is essential for viability in D. radiodurans. ... Gene silencing and large-scale ... pin board white companyWebFeb 1, 2024 · We recently characterized densely occupied, multi-kilobase regions in the E. coli genome that are transcriptionally silent, similar to eukaryotic heterochromatin. These … to prepare iron sulphide by heating a mixtureWebThese regions, which we refer to as extended protein occupancy domains (EPODs) following the nomenclature of Vora and colleagues [10], appear to act, at least partially, … to prepare and arrangeWebJul 31, 2009 · We performed a systematic search for these extended protein occupancy domains (EPODs) under early exponential growth using an automated algorithm that … pin board white