WebThirty novel 5-substituted benzyloxy-2-arylbenzofuran-3-carboxylic acids (B01-B30) were synthesized and evaluated for their TMEM16A inhibitory activity by using short circuit current measurements in Fischer rat thyroid (FRT) cells expressing human TMEM16A. IC(50) values were calculated using YFP fluorescence plate reader assay. WebSep 8, 2016 · (A) Fischer rat thyroid (FRT) cells stably expressing WT, F508del mutant, or P67L mutant CFTR from an identical genomic integration site were treated with 3 μM lumacaftor. After 24 hours, cells were lysed, and total cellular protein was analyzed by Western blotting with anti-human CFTR antibodies (10B6.2).
Influence of cell background on pharmacological rescue of …
WebSep 8, 2015 · Fisher rat thyroid (FRT) epithelial cells as a model as they have been shown to be suitable for studies of other epithelial ion channels including CFTR and epithelial Na channels at the level of single cells, as well as cell monolayers suitable for integrated studies of transepithelial ion transport (32, 33). FRT WebFischer Rat Thyroid (FRT) cells have been used extensively for studies of cystic fibrosis transmembrane conductance regulator (CFTR) protein for more than 20 years and have been a ”workhorse” for drug discovery in the disease. 1 The cells have been used by the … A biorepository is a place, similar to a library or bank, where biological samples are … brands who use benevity
Detergent-insoluble GPI–anchored Proteins Are …
WebFisher rat thyroid (FRT) cells were cultured in 6-well plates with Coon’s modified F12 medium and the addition of fetal bovine serum (10% FBS), 2.68 g/L sodium bicarbonate, 2 mM glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin. Antibiotics were removed before the addition of TRIDs. WebMar 5, 2001 · Fischer rat thyroid (FRT) cells (Lipardi et al. 1998), the gift of Dr. Enrique Rodriguez-Boulan (Cornell University, Ithaca, New York), were maintained in Coon's F12 media with 10% FBS. COS, CHO, and HEK 293 cells (American Type Culture Collection) were maintained in DME with 5% iron-supplemented calf serum or 10% FBS. WebWe chose Fischer rat thyroid (FRT) epithelia for two reasons. First, when grown on permeable filter supports, FRT cells form polarized epithelia with a high transepithelial … brandswitch