2024 Phee401质粒

Phee401质粒

Author: spyq

August undefined, 2024

WebpHEE401: Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance: Loading... 71287: pHEE401E: Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 ... WebUE质粒小量制备试剂盒. RNase A: 50 mg/ml, 室温可贮存6个月，长期贮存于-20℃。. Buffer S1: 细菌悬浮液。. 加入RNaseA后，混合均匀，4℃贮存。. Buffer S2: 细菌裂解液（含SDS/NaOH), 室温密闭贮存。. Buffer S3: 中和液，室温密闭贮存。. BufferW1: 洗涤液，室温密闭贮存。. Buffer W2 ...

pHT01载体质粒图谱、序列、说明书、价格_the - 搜狐

WebMar 11, 2024 · We thank Dr. Qijun Chen for sharing the plasmids pHEE401 and pCBC-DT1T2, Dr. Donald P. Weeks for sharing the vector pCAMBIA-Cas9. This work was supported by the Shenzhen Science and Technology Innovation Committee (JCYJ20240818100038326), Natural Science Foundation of Guangdong Province (2024A030313966), National Natural … http://www.biofeng.com/adjy/zhili/pBUE411.html sand sifting starfish size

分子克隆：从认识质粒开始 - 知乎 - 知乎专栏

WebSep 12, 2024 · 做分子实验，经常和不同的质粒打交道，了解各种质粒的图谱信息是必需的，今天就给大家介绍一下怎么查找质粒图谱。 WebPartial Sequences from Addgene (3) Addgene has sequenced portions of this plasmid for verification. The results are shown below. Leading primers are indicated on the first line of … http://www.biofeng.com/adjy/zhili/pHSN401.html sand sifting starfish dying

Phee401质粒

WebpHSE401质粒价格1200元，pHSE401(爱迪基因 62201 )质粒图谱（map），质粒序列(Sequence)见下文，质粒抗性为卡那霉素，质粒大小为6500 bp，测序引物为Spect-F … WebApr 25, 2024 · 质粒（英语：Plasmid），又名质体，是指在细胞的染色体或核区DNA之外，能够自主复制的DNA分子（字源：plasm为生殖质，-id表示粒）。质粒与染色体最主要的区分是，质粒不是细胞生存所必需，染色体则是细胞生存必需的。大部分的质粒都是环状分子，但是也有少数属于线状分子，它存在于许多细菌 ...

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http://www.bioengx.com/%e7%8c%ae%e7%bb%99%e5%88%9d%e5%ad%a6%e8%80%85%ef%bc%9a%e5%a6%82%e4%bd%95%e9%ab%98%e6%95%88%e6%9f%a5%e6%89%be%e8%b4%a8%e7%b2%92%e5%9b%be%e8%b0%b1/ WebpHEE401 (~100 ng/μl) 2 μl 10× T4 DNA Ligase Buffer (NEB) 1.5 μl 10× BSA 1.5 μl BsaI (NE B) 1 μl T4 DNA Ligase (HC, NEB) 1 μl ddH 2 O 6 μl Total volume 15 μl 5. Transform E.coli competent cells with 5 μl of reaction mixture, and select …

WebPlasmid pHSN401 from Dr. Qi-Jun Chen's lab contains the inserts 3×FLAG-NLS-zCas9-NLS and gRNA scaffold and is published in BMC Plant Biol. 2014 Nov 29;14(1):327. This plasmid is available through Addgene. WebpHSE401质粒. 当前位置: 首页 > 质粒载体 > 其他 > pHSE401质粒. pHSE401质粒. ￥799元. (会员等级价 ) ￥0元. 发货方式：. 质粒发货菌液发货.

Web1、质粒: 质粒是一种独立于宿主细胞染色体以外，能独立自主复制的遗传单位。. 它们常见的形式是细菌中环状的双链DNA分子，有的也存在于古细菌和真核生物中，其大小在1～200kb不等。. 质粒是“裸”DNA，不编码必要的基因，在自然界中，质粒通常携带有利于有 … Web如何订购质粒 Addgene offers specialized service to scientists in China through a partnership with a Chinese distributor, Beijing Zhongyuan.

Web双链抗体表达载体构建 1，空载质粒准备pEE 12.4(主)或者pXC17.4，提供含有复制子、原核抗性、GS抗性等主要骨架，如果表达的抗体是单链只需要这个主要载体就可以； pEE6.4(辅助) 或者pXC18.4，只是提供抗体轻链的：…

Web阴离子交换色谱法 (AEX)根据分析物在表面负电荷数量和定位方面的差异分离分析物。. 业界普遍认为，AEX是一种稳定、可重现、易于自动化的分析技术，且只需少量样品。. 由于质粒DNA因骨架上的磷酸基团而带负电荷，因此AEX是用于质粒相关分离和分析的不二之选 ... sand sifting starfish careWeb阴离子交换色谱法 (AEX)根据分析物在表面负电荷数量和定位方面的差异分离分析物。. 业界普遍认为，AEX是一种稳定、可重现、易于自动化的分析技术，且只需少量样品。. 由于 … shore meadows lake george nyWebA. 用50ul无血清培养基稀释质粒DNA，轻轻混匀。B. 取适量合适的质粒转染试剂在50ul无血清培养基中稀释，室温孵育5min。C. 将前两步所稀释的DNA和质粒转染试剂混合，轻轻混匀，室温放置30min。. 3、在每孔细胞中加入混合后的转染液，轻轻摇匀。. 4、贴壁细胞可在 … sands ii carolina beachWebPlasmid pHSE401 from Dr. Qi-Jun Chen's lab contains the inserts gRNA scaffold and zCas9 and is published in BMC Plant Biol. 2014 Nov 29;14(1):327. This plasmid is available through Addgene. shore mckinley conger \\u0026 jolley llphttp://www.vectorhub.cn/difference-between-plasmid-vector.html shore meadow silecroftWebpKSE401质粒价格1200元，pKSE401(爱迪基因#62202 )质粒图谱（map），质粒序列(Sequence)见下文，质粒抗性为卡那霉素，质粒大小为6500 bp，测序引物为Spect-F。pKSE401质粒是植物表达；植物二元向量表达。 sand silt and clay particlesWebPlasmid pHEE401 from Dr. Qi-Jun Chen's lab contains the inserts sgRNA scaffold and zCas9 and is published in Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. … sands iii carolina beach nc