Putative endolysin
WebAug 6, 2016 · Endolysin from Staphylococcus aureus phage SA97 (LysSA97) was cloned and investigated. LysSA97 specifically lyse the staphylococcal strains and effectively disrupted staphylococcal biofilms. Bioinformatic analysis of LysSA97 revealed a novel putative cell wall binding domain (CBD) as well as two enzymatically active domains …
Putative endolysin
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WebOct 7, 2014 · Resistance rates are increasing among several problematic Gram-negative pathogens, a fact that has encouraged the development of new antimicrobial agents. This … WebMar 15, 2024 · Bioinformatics analysis using BLAST with LysK endolysin gene sequence as query allowed for the identification of 25 putative endolysin encoding genes. Cg and …
WebAn open reading frame homologous to the genes encoding several cell-wall hydrolyzing enzymes was identified on the genome of actinophage μ1/6. This open reading frame encoding the putative endolysin was amplified by polymerase chain reaction and cloned into the expression vector pET-21a. This gene consisted of 1182 bp encoding a 393 amino … WebSep 1, 2024 · Identification and overexpression of recombinant endolysin LysSTG2. From the genome sequence of phage STG2, the predicted open reading frame for a putative endolysin gene encoding a protein (LysSTG2) consisting of 138 amino acids (expected molecular mass of 15.3 kDa) was identified.
WebEndolysin is the term for muralytic enzymes that degrade the cell wall; endolysins accumulate in the cytosol fully folded during the vegetative cycle. ... Holins constitute one of the most diverse functional groups, with >100 known or putative holin sequences, which form >30 ortholog groups. Publication types Research Support, Non-U ... WebJan 31, 2024 · Putative endolysin 117 was the only successfully overexpressed endolysin, and it was renamed LyJH1892. LyJH1892 showed potent lytic activity against both methicillin-susceptible S. aureus and MRSA and showed broad lytic activity against coagulase-negative staphylococci.
WebNov 13, 2013 · The putative endolysin gene of IME-EF1 was cloned and fused with His tag sequence into vector pColdI. Through the induction of 1 mM isopropyl beta-D-thiogalactoside (IPTG), the transformed E. coli M15 expressed 30 kDa soluble protein, and the band can be probed using anti-His Tag antibody .
WebApr 12, 2011 · We found that an endolysin gene (psm) in phiSM101, an episomal phage of enterotoxigenic C. perfringens type A strain, SM101 (Myers et al. 2006), encodes a … ksp nasa countdown won\\u0027t resizeWebMay 25, 2024 · Bacteriophage encoded endolysin as an alternative antibiotics is a peptidoglycan degrading enzyme with a low rate of resistance. Here, the engineered endolysin was developed to defeat multiple drug-resistant (MDR) Acinetobacter baumannii. First, putative endolysin PA90 was predicted by genome analysis of isolated … ksp near future mod downloadWebApr 10, 2024 · As a result, any dynamic endolysin-based infection power merchants must combine outer-membrane-permeabilizing marketers after remaining ... Wang G et al (2024) Biological properties and genomics analysis of vB_KpnS_GH-K3, a Klebsiella phage with a putative depolymerase-like protein. Virus Genes 55:696–706. Article CAS PubMed ... ksp near future thermalWebJul 21, 2024 · In this study, an open reading frame encoding putative endolysin was cloned from the complete genome data and expressed in the Escherichia coli expression system. … ksp north kerbin dynamics renewedWebThe 723 putative endolysins ranged from 72 to 578 residues in length, using 24 different catalytic domains and 18 different cell wall-binding domains organized with 89 different … ksp not in full screenWebFeb 15, 2024 · The gene (GenBank accession no. OL415943) encoding putative endolysin from bacteriophage PBEC131 was cloned into pET21a+ (Novagen, United States) using … ksp nullreferenceexceptionWebDec 9, 2024 · Here we introduce a putative phage lysin that specifically lyses vegetative C. botulinum Group I cells. This lysin, called CBO1751, efficiently kills cells of C. botulinum Group I strains at the ... kspn short interest